rnase h (New England Biolabs)
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New England Biolabs
rnase h
Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+h/RNase+H/pmc12926610-373-32-34
Average 99 stars, based on 5317 article reviews
Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+h/RNase+H/pmc12926610-373-32-34
Average 99 stars, based on 5317 article reviews
rnase h - by Bioz Stars,
2026-09
99/100 stars
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Reverse Transcription:Article Title: Macrophage MERTK restrains GRAMD1A-driven mitochondrial oxysterol trafficking to limit colitis Article Snippet: Poly(A) RNA was purified from 1 μg total RNA using Dynabeads Oligo(dT) (Thermo Fisher) through two rounds of purification, followed by fragmentation with the Magnesium RNA Fragmentation Module (NEB) at 94 °C for 5-7 min. .. The fragmented RNA was reverse-transcribed into cDNA using SuperScriptTM II Reverse Transcriptase (Invitrogen) and subsequently used to synthesize U-labeled second-strand DNA with E. coli DNA polymerase I, Article Title: Patient-Derived Lung Cancer Organoids for Precision Oncology: A Comprehensive Analysis Article Snippet: After purification, the mRNA was fragmented into short fragments using divalent cations under elevated temperature (Magnesium RNA fragmentation Module [cat.e6150] [New England Biolabs (NEB), Ipswich, MA] under 94°C 5-7 min). .. Then the cleaved RNA fragments were reverse-transcribed to create the circulating DNA by SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649 [ThermoFisher]), which were next used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat.m0209, NEB), Article Title: From disease model to therapeutic insight: An engineered hydrogel reveals the role of matrix viscous dissipation in intervertebral disc degeneration Article Snippet: .. The resulting RNA fragments were reverse-transcribed to create cDNA using SuperScriptTM Reverse Transcriptase (Invitrogen, USA), which was subsequently used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, USA), Article Title: Screening of M6a methylated genes associated with preovulatory follicle development in Bashang long-tailed chicken Article Snippet: The cleaved RNA fragments were incubated for 2 h at 4°C with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630). .. The immunoprecipitated RNA was reverse-transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), followed by synthesis of U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat. m0209, USA), Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion Article Snippet: Following purification, the mRNA was fragmented into short fragments using divalent cations under elevated temperature [Magnesium RNA Fragmentation Module (cat. no. e6150; New England BioLabs, Inc.) under 94°C 5-7 min]. .. Then the cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScript II Reverse Transcriptase (cat. no. 1896649; Invitrogen; Thermo Fisher Scientific, Inc.), which were next used to synthesized U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat. no. m0209; New England BioLabs, Inc.), cDNA Synthesis:Article Title: Integrated transcriptomic, proteomic, and metabolomic analyses reveal molecular mechanisms underlying clutch length differences in goose ovaries Article Snippet: The purified mRNA was then fragmented and reverse-transcribed into first-strand cDNA using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, MA, USA). .. Second-strand cDNA synthesis was performed incorporating dUTP (Thermo Fisher Scientific, MA, USA), DNA Polymerase I ( E. coli ) (New England Biolabs, MA, USA), and Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet Article Snippet: First-strand cDNA synthesis was conducted with random primers and Superscript II Reverse Transcriptase (catalog no. 18064014, Invitrogen, Carlsbad, CA, USA). .. Subsequently, second-strand cDNA synthesis was performed, employing DNA Polymerase I and Immunoprecipitation:Article Title: Screening of M6a methylated genes associated with preovulatory follicle development in Bashang long-tailed chicken Article Snippet: The cleaved RNA fragments were incubated for 2 h at 4°C with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630). .. The immunoprecipitated RNA was reverse-transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), followed by synthesis of U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat. m0209, USA), Synthesized:Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion Article Snippet: Following purification, the mRNA was fragmented into short fragments using divalent cations under elevated temperature [Magnesium RNA Fragmentation Module (cat. no. e6150; New England BioLabs, Inc.) under 94°C 5-7 min]. .. Then the cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScript II Reverse Transcriptase (cat. no. 1896649; Invitrogen; Thermo Fisher Scientific, Inc.), which were next used to synthesized U-labeled second-stranded DNAs with E. coli DNA polymerase I (cat. no. m0209; New England BioLabs, Inc.), |